Sulfurous Analysis of Bioelectricity Generation from Sulfate-reducing Bacteria (SRB) in a Microbial Fuel Cell

Document Type: Research Paper

Authors

1 PhD Student, University of Science and Technology Tehran, Iran

2 Faculty of Chemical Eng. Tarbiat Modares University, Tehran, Iran

3 Faculty of Chemical Eng., Tarbiat Modares Univ. Tehran, IRan

4 Department of Chemical Engineering, Biotechnology group, Amirkabir University of Technology, Tehran, Iran

5 Microbiology Group, Shahed University, Tehran, Iran

Abstract

The current importance of energy emphasizes the use of renewable resources (such as wastewater) for electricity generation by microbial fuel cell (MFC). In the present study, the native sulfate-reducing bacterial strain (R.gh 3) was employed simultaneously for sulfurous component removal and bioelectricity generation. In order to enhance the electrical conductivity and provision of a compatible bed, a complex electrode structure based on stainless steel-304 was prepared. Next, the electrode was coated with a composite of graphite and activated carbon solution. A new approach associated with increasing bacterial population was studied using two electron acceptors composed of iron and sulfate for respiration of sulfate-reducing bacteria. Finally, according to the maximum living cell number (nM = 20 ´108 cell ml-1) and the conditions of the bioreactor including the highly efficient anode electrode, a higher current generation (2.26 mA for the new structure as compared to 1.73 and 1.29 mA for graphite rod and carbon paper, respectively) was observed in the culture media.

Keywords

Main Subjects


[1] Rezaei F, Xing D, Wagner R, Regan JM, Richard TL, Logan BE., "Simultaneous cellulose degradation and electricity production by Enterobacter cloacae in a microbial fuel cell", Appl. Environ. Microbiol., 2009,75:3673.

[2] Fraiwan A, Mukherjee S, Sundermier S, Lee H-S, Choi S., "A paper-based microbial fuel cell: Instant battery for disposable diagnostic devices", Biosens. Bioelectron., 2013,49:410.

[3] Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, et al., "Microbial fuel cells: methodology and technology", Environ. Sci. Technol., 2006,40:5181.

[4] Liu H, Ramnarayanan R, Logan BE., "Production of electricity during wastewater treatment using a single chamber microbial fuel cell", Environ. Sci. Technol., 2004,38:2281.

[5] Gibson GR, Macfarlane G, Cummings J., "Sulphate reducing bacteria and hydrogen metabolism in the human large intestine", Gut., 1993,34:437.

[6] Odom J., "Industrial and Environmental concerns with sulfate-reducing bacteria", ASM News (Washington), 1990,56:473.

[7] Ghazy E, Mahmoud M, Asker M, Mahmoud M, Abo Elsoud M, Abdel Sami M., "Cultivation and detection of sulfate reducing bacteria (SRB) in sea water", J. of American Sci., 2011,7:604.

[8] Otero JJG., "Epidemiology of marine toxins", Seafood and Freshwater Toxins: Pharmacology, Physiology, and Detection, 2014:123.

[9] Peng C-G, Park JK, Patenaude RW., "Statistics-based classification of microbially influenced corrosion in freshwater systems", Water Res., 1994,28:95.

[10] Gerardi MH., "Sulfur‐Oxidizing and Sulfur‐Reducing Bacteria", Wastewater Bacteria, 2006,117:31.

[11] Nordstrom DK., "The effect of sulfate on aluminum concentrations in natural waters: some stability relations in the system Al2O3-SO3-H2O at 298 K", Geochim. Cosmochim. Acta, 1982,46:681.

[12] Sun M, Tong Z-H, Sheng G-P, Chen Y-Z, Zhang F, Mu Z-X, et al., "Microbial communities involved in electricity generation from sulfide oxidation in a microbial fuel cell", Biosens. Bioelectron., 2010,26:470.

[13] Zhao F, Rahunen N, Varcoe JR, Chandra A, Avignone-Rossa C, Thumser AE, et al., "Activated carbon cloth as anode for sulfate removal in a microbial fuel cell", Environ. Sci. Technol., 2008,42:497.

[14] Clauwaert P, Aelterman P, De Schamphelaire L, Carballa M, Rabaey K, Verstraete W., "Minimizing losses in bio-electrochemical systems: the road to applications", Appl. Microbiol. Biotechnol., 2008,79:901.

[15] Logan BE., "Exoelectrogenic bacteria that power microbial fuel cells", Nat. Rev. Microbiol., 2009,7:375.

[16] Zhou M, Chi M, Luo J, He H, Jin T., "An overview of electrode materials in microbial fuel cells", J. Power Sources., 2011,196:4427.

[17] Hindatu Y, Annuar M, Gumel A., "Mini-review: Anode modification for improved performance of microbial fuel cell", Renewable Sustainable Energy Rev., 2017,73:236.

[18] Jia N, Wang Z, Yang G, Shen H, Zhu L., "Electrochemical properties of ordered mesoporous carbon and its electroanalytical application for selective determination of dopamine", Electrochem. Commun., 2007,9:233.

[19] Postgate J., "Differential media for sulphur bacteria", J. Sci. Food Agric., 1959,10:669.

[20] Nevin KP, Richter H, Covalla S, Johnson J, Woodard T, Orloff A, et al., "Power output and columbic efficiencies from biofilms of Geobacter sulfurreducens comparable to mixed community microbial fuel cells", Environ. Microbiol., 2008,10:2505.

[21] Stadler R, Fuerbeth W, Harneit K, Grooters M, Woellbrink M, Sand W., "First evaluation of the applicability of microbial extracellular polymeric substances for corrosion protection of metal substrates", Electrochimica Acta, 2008,54:91.

[22] McKane L, Kandel J., "Bacterial Growth and Laboratory Cultivation", Microbiology Essentials and Applications New York: McGraw-Hill, Inc., 1996,97:125.

[23] Cord-Ruwisch R., "A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria", J. Microbiol. Methods, 1985,4:33.

[24] Karamanev D, Nikolov L, Mamatarkova V., "Rapid simultaneous quantitative determination of ferric and ferrous ions in drainage waters and similar solutions", Miner. Eng., 2002,15:341.

[25] Zhao F, Wu X, Wang M, Liu Y, Gao L, Dong S., "Electrochemical and bioelectrochemistry properties of room-temperature ionic liquids and carbon composite materials", Anal. Chem., 2004,76:4960.

[26] Aelterman P, Versichele M, Marzorati M, Boon N, Verstraete W., "Loading rate and external resistance control the electricity generation of microbial fuel cells with different three-dimensional anodes", Bioresour. Technol., 2008;99:8895.

[27] Rabaey K, Verstraete W., "Microbial fuel cells: novel biotechnology for energy generation", Trends Biotechnol., 2005,23:291.

[28] Herrera LK, Videla HA., "Role of iron-reducing bacteria in corrosion and protection of carbon steel", Int. Biodeterior. Biodegrad., 2009,63:891.

[29] Lopes F, Morin P, Oliveira R, Melo L., "Interaction of Desulfovibrio desulfuricans biofilms with stainless steel surface and its impact on bacterial metabolism", J. Appl. Microbiol., 2006,101:1087.

[30] Lovley DR, Coates JD, Blunt-Harris EL, Phillips EJ, Woodward JC., "Humic substances as electron acceptors for microbial respiration" Nature, 1996,382:445.

[31] Lovley DR, Phillips EJ., "Competitive mechanisms for inhibition of sulfate reduction and methane production in the zone of ferric iron reduction in sediments", Appl. Environ. Microbiol., 1987,53:2636.

[32] Castro HF, Williams NH, Ogram A., "Phylogeny of sulfate-reducing bacteria", FEMS Microbiology Ecology, 2000,31:1.

[33] Hashemi J, Samimi A., "Steady state electric power generation in up-flow microbial fuel cell using the estimated time span method for bacteria growth domestic wastewater", Biomass and bioenergy, 2012,45:65.

[34] Singh R, Kumar A, Kirrolia A, Kumar R, Yadav N, Bishnoi NR, et al., "Removal of sulphate, COD and Cr (VI) in simulated and real wastewater by sulphate reducing bacteria enrichment in small bioreactor and FTIR study", Bioresour. technol., 2011,102:677.

[35] Song YC, Kim DS, Woo JH, Subha B, Jang SH, Sivakumar S., "Effect of surface modification of anode with surfactant on the performance of microbial fuel cell", Int. J. Energy Res., 2015,39:860.

[36] Hou J, Liu Z, Yang S, Zhou Y., "Three-dimensional macroporous anodes based on stainless steel fiber felt for high-performance microbial fuel cells", J. Power Sources, 2014,258:204.

[37] Zheng S, Yang F, Chen S, Liu L, Xiong Q, Yu T, et al., "Binder-free carbon black/stainless steel mesh composite electrode for high-performance anode in microbial fuel cells", J. Power Sources, 2015,284:252.

[38] Guo K, Donose BC, Soeriyadi AH, Prévoteau A, Patil SA, Freguia S, et al., "Flame oxidation of stainless steel felt enhances anodic biofilm formation and current output in bioelectrochemical systems", Environ. Sci. Technol., 2014,48:7151.

[39] Lamp JL, Guest JS, Naha S, Radavich KA, Love NG, Ellis MW, et al., "Flame synthesis of carbon nanostructures on stainless steel anodes for use in microbial fuel cells", J. Power Sources, 2011,196:582.

[40] Erbay C, Pu X, Choi W, Choi M-J, Ryu Y, Hou H, et al., "Control of geometrical properties of carbon nanotube electrodes towards high-performance microbial fuel cells", J. Power Sources, 2015,280:347.